Nested RT-PCR method for the detection of European avian-like H1 swine influenza A virus

Detection of avian influenza virus of H9 subtype in the feces of experimentally infected chickens by RT–PCR

  Avian Influenza (AI) is a viral respiratory disease of domestic and wild birds. In the diagnostic laboratory, it is essential to have methods for rapid detection of avian respiratory viruses. Cloacal swabs collected from chickens experimentally infected with H9 subtype AI virus, used in a reverse transcription-polymerase chain reaction (RT-PCR) assay for detection of AI. In infected animals, ...

the use of a sensitive rt-nested pcr method for detection of hepatitis c virus

objective: hepatitis c virus is the major cause of viral hepatitis and its diagnosis in suspected specimens is of great importance. the risk of transfusion- transmitted virus infection is primarily the result of failure in serological screening tests to detect recently infected donors in the pre-seroconversion window period of infection. therefore, sensitive and accurate diagnosis of hcv prior ...

Standardization of ELISA for Detection of Avian Influenza Virus Antibodies

A Reverse transcription-PCR assay for detection of type A influenza virus and differentiation of avian H7 subtype

Abstract : Avian influenza virus (AIV) infection is a major cause of influenza mortality in birds and can cause human mortality and morbidity. Although the risk of infection with avian influenza virus (AIV) is generally low for most people, the pathogenic virus can cross the species barrier and acquires the ability to infect and be transmitted among the human population; therefore the ra...

Immunofluorescence Method for The Detection of Non-Pathogenic Influenza Virus

The direct F.A. Method has been used for diffe­rentiation of virulent and avirulent lines of influenza viruses. A positive fluorescence was obtained indi­cating the level of virus in the tissues, sorbing with dried mouse powder helped to reduce non-specific staining. The virulent line showed the greatest num­ber of fluorescent cells with the peak at 10 hours. the non-virulent lines showed a gra...